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大麦干种子DNA提取研究

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进行了应用改进后CTAB方法从半粒大麦种子中提取DNA的研究.结果表明,从干种子中提取的DNA是完整的,多糖等大分子及RNA也被去除;提取的DNA与大麦叶片中提取的DNA是一致的;其PCR扩增产物与从叶片中提取的DNA扩增产物有相同的条带类型.
Study on DNA Extraction in Dry Barley Seed
DNA was extracted from barley half seed using modified CTAB method.Result showed that the DNA extracted from dry seed was complete, which could effectively remove RNA and macromolecular. It showed the same banding patterns with those from leaf DNA.Amplification products of PCR from leaves had the same brand.

Half seedDNA extractionCTAB methodMolecular marker

王芳

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青海省农林科学院,青海西宁,810016

半粒种子 DNA提取 CTAB法 分子标记

2007

安徽农业科学
安徽省农业科学院

安徽农业科学

CSTPCDCSCD北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2007.35(26)
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