首页|NaCl胁迫对海滨木槿种子萌发及Na+、K+含量的影响

NaCl胁迫对海滨木槿种子萌发及Na+、K+含量的影响

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[目的]探索不同浓度NaCl处理对海滨木槿种子萌发及种皮和种胚中Na+、K+含量的影响.[方法]用NaCl浓度分别为0、50、100、150、200 mmol/L NaCI的1/4 Hoagland营养液培养材料,培养5、10 d后以FP-640火焰分光光度计测定种皮和胚中的Na+、K+含量;培养15 d后,将含有Nacl的营养液未萌发的种子分别转入1/4Hoagland培养液继续培养,测定解除盐胁迫后种子萌发的恢复率.[结果]随着培养液中NaCI浓度的升高,海滨木槿种子的萌发率逐渐降低,在NaCI浓度为200mmol/L时种子的萌发率最低.种皮和胚中Na+含量随着培养液中NaCl浓度的升高和处理天数的延长而增大;在相同的盐浓度下,胚中Na+舍量明显低于种皮.随着培养液中NaCl浓度的升高,种皮中K+含量逐渐升高,胚中K+含量逐渐降低,但胚中K+含量明显高于种皮.种皮和胚中Na+/K+随着NaCI浓度的升高而增大,胚中Na+/K+明显低于种皮.[结论]海滨木槿的种皮是阻挡Na+进入种子的一道屏障;NaCI处理下胚保持较高含量的K+和较低的Na+/K+可能是海滨木槿种子耐盐的主要原因之一.
Effects of NaCI Stress on the Germination and the Content of Na+ and K+of Hibiscus hamabo Sieb.et Zucc.Seeds
[Objective]The research aimed to discuss the effects of salt treatment with different concentrations NaCl on the seed germination and the content of Na+ and K+ in seed coat and embryo of Hibiscus hamabo Sieb et Zucc. [Method]The materials were cultivated by 1/4 Hoagland nutrition solution with 0, 50, 100, 150, 200 mmol/L NaCl. the content of Na + and K+ in seed coat and embryo was determined by frame spectrophotometer-640 when the seeds were treated for 5 days and todays. After 15 days, The no germination seeds in 1/4 Hoagiand ntt nutrition solution with Na were cultivated by 1/4 Hoagiand nutrition solution without NaCl to study the recovery rate of germination.[Rcsult]The results showed that the seed germination rate decreased gradually as the NaCl concentration increased and it was lowest when the seeds were treated with 200 mmol/L NaCl. The Na+ content of the seed coat and embryo increased gradually as the NaCl concentration increased and days progressed, the Na+ content of the embryo was significantly lower than that of seed coat under the same NaCl concentration. As the NaCl concentration increased, the K+ content of the seed coat increased gradually while it decreased gradually in the embryo, but the latter was significantly higher than the former. The Na+/K+ of the seed coat and embryo increased gradually as the NaCl concentration increased, and the Na+/K+ of the embryo was significantly lower than that of the seed coat, [Conelusion]The seed coat of Hibiscus hamabo Sieb.et Zucc. could prevent Na+ into the seed and the higher K+ and the lower Na+/K+ in the embryo could be one of key reasons of high-salinity resistance.

Hibiscus hamabo Sieb. et ZuccSalt stressSeed germinationNa+ and K+ content

薄鹏飞、孙秀玲、宋杰、杜希华、薛庆荣

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山东师范大学生命科学学院,山东,济南,250014

海滨木槿 盐胁迫 种子萌发 Na+、K+离子含量

山东师范大学博士启动基金

2003-10

2008

安徽农业科学
安徽省农业科学院

安徽农业科学

CSTPCD北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2008.36(8)
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