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玉米皮多糖的制备方法及结构研究

Study on the Preparation Method and Structure of Polysaccharide in Corn Skin

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[目的]研究玉米皮中水溶性多糖的制备方法和化学结构.[方法]采用单因素试验研究了多糖的最佳提取工艺,采用高效液相色谱、红外光谱、紫外光谱对多糖结构进行了解析.[结果]玉米皮多糖的最佳提取工艺为:料水比1:30,在100℃提取2次,每次2h.在该提取条件下,100g玉米皮可制备得到6.94g多糖.多糖纯度为88.18%.玉米皮多糖主要由α-D-吡喃葡萄糖组成.其含量为90.8%.此外,玉米皮多糖还含有少量的阿拉伯糖、木糖、半乳糖等单糖以及微量的蛋白质.[结论]玉米皮中水溶性多糖含量较高.有很好的开发利用价值.
[Objective] The research aimed to study the preparation method and structure of water-soluble polysaccharide in corn skin (PCS). [Method] The optimum extracted condition of polysaccharide was studied by using one-factor experiment and the structure of polysaccharide was analyzed by using HPLC, IR spectrum and UV spectrum. [Result] The optimum extracted conditions were that material: water was 1:30, temperature was 100℃, extracted time was 2 h, extracted times was 2 times. From 100 g corn skin, 6.94 g polysaccharide could be obtained according to the above conditions, and the purity was 88.18%. The main sugar of the polysaccharide was α-D-Glup (90.8%), along with small arabinose(Ara), xylose(Xyl), galactose(Gal)and protein. [Conclusion] Corn skin contained quite a few water-soluble polysaccharide, so it had high value of use and development.

Corn skinPolysaccharidePreparationStructure

芦菲、李波、李春阳、戴军

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河南科技学院食品学院,河南新乡,453003

江苏省农业科学院农产品加工研究所,江苏南京,210014

江苏省江南大学分析测试中心,江苏无锡,214036

玉米皮 多糖 制备 结构

河南省高等学校新世纪优秀人才支持计划江苏省农业科学院资助项目

2006HANCET-176110648

2008

安徽农业科学
安徽省农业科学院

安徽农业科学

CSTPCD北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2008.36(17)
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