Analysis on Influence of Several Factors on Rapid Propagation of Shoot Tip of Strawberry
[Objective] The study was to analyze the several factors of influenced the rapid propagation of shoot tip of strawberry. [Method] With strawberry variety Tongzi 1 as tested material, the factors influencing the size of material, growth regulators, carbon source in rapid propagation of shoot tip of strawberry were analyzed. [Result] The virus elimination rate of inoculation with 0.5 mm shoot tip was highest and its survival rate was roost ideal. The optimum medium and best multiplication medium of induction shoot tip survival and growth of strawberry Tongzi tall was MS+ BA 0.5 mg/L. When the concn. of IBA was 800 mg/L, the in vitro rooting of tissue culture seedling not only had high rooting rate and root number, but also obtained stronger rooting seedlings. With sucrose and sugar as carbon source, the propagation coefficient was ideal and that of sugar was optimum. [Conclusion] The rapid propagation of shoot tip of strawberry should use 0.5 run shoot tip as material. The differentiation and subculture medium was MS + ISA 0.5 mg/L. The optimum growth regulator for soaking the root of tissue culture seedling was 800 mg/L IBA.
国家科技期刊平台
NSTL
万方数据
维普
