Genetic Polymorphism of 18 Lycium barbarum Resources Based on nrDNA ITS Sequences
[Objective] The study aimed to investigate the genetic polymorphism of 18 Lycium barbarum resources via nrDNA ITS sequencing. [Method]The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB method for PCR amplification on the nrDNA ITS region using specifically synthesized primers; the amplified fragments were cloned and sequenced, then the sequencing results were clustered. [Result] nrDNA ITS sequences of the tested 18 Lycium barbarum were firstly obtained in the present study. For whole 18 tested materials, the variation range of whole ITS region is 559-643 bp, with an average of 612 hp; alignment analyses showed that the whole length of internal transcribed spacer (ITS1 + ITS2) was 480 bp, within which there are 194 variation sites, accounting for 40.4% , and 286 conserved sites, accounting for 59.6% , The cluster results showed that the 18 tested materials could be grouped into three classes. [Conclusion] Analysis of nrDNA ITS sequence may avail to identity the Lycium barbarum germplasm resources.
国家科技期刊平台
NSTL
万方数据
维普
