Study on the Isolation of Endophytic Fungi from the Plants Grown in Alpine Grassland and Its Antifungal Activity
[Objective] The aim was to study the diversity of endophytic fungi isolated from four alpine grassland plants and screen out the strains with antifungal activities. [Method] By using tissue isolation method, endophytic fungi were isolated from different tissues of Kobresia capillifolia (Decne. ) C.B. Clarke, Elymus dahuricus Turcz. , Carex heterastachys Bunge and Achnatherum inebrians (Hance) Keng collected in the alpine grassland. With Rhizoctonia solani (short for R-1), Bipolaris sorokiniana (short for B-1), Pythium ultimum (short for P-1) and Fusarium oxysporium (short for F-1) as test fungi, the antifungal activities of the isolated endophytic fungi were screened out. [Result] From 4 kinds of plants, 45 different endophytic fungal strains were isolated. Among them, 38 strains have antifungal activities against at least one pathogen. And two of them had strong inhibition on Rhizoctonia solani, while thirteen of them had strong inhibition on Bipolaris sorokiniana. Their inhibition on Fusarium oxysporium was very common but notstrong, and only one strain has strong inhibition on Pythium ultimum. [Conclusion] The endophytic fungi strains with antifungal activity were obtained in this study.
国家科技期刊平台
NSTL
万方数据
维普
