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半夏中总生物碱及鸟苷含量动态变化研究

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[目的]对半夏中总生物碱及鸟苷的含量变化进行动态研究,为半夏的合理采收及GAP种植提供科学依据.[方法]分别采用分光光度法和高效液相色谱法对贵州不同产地、不同采收季节、不同海拔高度半夏中总生物碱、鸟苷进行含量测定.[结果]半夏中总生物碱范围在0.009 0%~0.032 0%,鸟苷范围在0.000 4%~0.024 0%,贵州威宁县10月采收的半夏总生物碱及鸟苷含量最高.[结论]该研究方法简单快速,结果可靠,可作为GAP种植半夏药材的质量控制方法.
Dynamic Determination of Alkaloid Content and Guanosine in Rhizoma Pinellia rernata
[Objective] The study aimed to provide scientific basis for reasonable harvest and GAP plant of Pinellia rernata by dynamic determination of its alkaloid content and guanosine. [Method] Using methods of UV and HPLC to determine alkaloid content and guanosine in Rhizoma Pinellia rernata at different places, seasons and heights. [Result] The range of alkaloid content in Pinellia ternat was 0.009 0%-0.032 0%. The range of guanosine was 0.000 4%-0.024 0%. The contents of the two chemical compositions were the highest on October. [Conclusion] This method has good reproducibility. It can be used for quality control of GAP plant for Rhizoma Pinellia ternate.

Pinellia ternateAlkaloid contentGuanosineUVHPLC

陈华国、汤洪波、周欣、胡晓娜

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贵州师范大学天然药物质量控制研究中心,贵州贵阳,550001

贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳,550001

半夏药材 总生物碱 鸟苷 UV HPLC

贵州省高层次人才科研条件特助经费项目贵州省中小企业局平台建设项目

TZJF-2006-26黔中小发[2008]76号

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2010.38(3)
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