[Objective] The research aimed screen the suitable condition of callus induction of Asarum heterotropoides Fr. Schmidt var. Mandshuricum Kitag and Asarum sieboldi Miq. Var. Seoulense Nakai. [Method] The different explants of Asarum heterotropoides Fr. Schmidt var. Mandshuricum Kitag and Asarum sieboldi Miq. Var. Seoulense Nakai were selected. The extraneous hormone of different allocated consistency proportion was added to the different culture medium for induction. [Result]The best explants of Asarum heterotropoides Fr. Schmidt var. Mandshuricum Kitag and Asarum sieboldi Miq. Var. Seoulense Nakai were their rhizome. The most suitable culture medium of Asarum heterotropoides Fr.Schmidt var.mandshuricum Kitag was MS+0.60 mg/L 6-BA + 0.15 mg/L NAA, and the most suitable culture medium of Asarum sieboldi Miq.var.seoulense Nakai was MS+0.60 mg/L 6-BA +0.10 mg/L NAA. [Conclusion] The study provided theoretic guidance for the induction of plant regeneration, scale production, the cultivation and promotion of new variety.
Asarum heterotropoides Fr. Schmidt var. mandshuricum Kitag.Asarum sieboldi Miq. var. seoulense NakaiCallusInduction
国家科技期刊平台
NSTL
万方数据
维普
