首页|安徽产委陵菜属7种药用种类的黄酮提取与分析

安徽产委陵菜属7种药用种类的黄酮提取与分析

扫码查看
采用正交设计研究了4因素4水平的委陵菜黄酮提取工艺条件,从最适样品浓度、上样量、乙醇洗脱液浓度、乙醇洗脱液用量等方面对AB-8型大孔树脂纯化委陵菜黄酮的工艺条件进行了研究.并用优选的提取条件提取了安徽产的7种药用委陵菜的黄酮,用比色法测定各种委陵菜的黄酮含量.结果得出,委陵菜黄酮在70 ℃条件下,乙醇浓度为40%,固液比为1: 100,提取3次的效果最佳.在该条件下,粗黄酮粉的黄酮含量为17.98%,经AB-8型大孔吸附树脂纯化后的委陵菜精黄酮粉中黄酮含量约达33.30%.
Extraction and Analysis of Flavonoids from Seven Herbal Species of Potentillae from Anhui Province
The technological conditions of extraction and purification of flavonoids from Potentilla chinensis from Anhui Province were studied by using 4-factor and 4-level orthogonal test. The technological conditions of flavonoids from Potentilla chinensis purified with AB-8 macroporous resin were studied from the aspects of the optimum sample concentration, load volume, the concentration of ethanol elution solution and ethanol elution volume. And flavonoid was extracted from 7 herbal species of Potentillae from Anhui Province by using the optimized extraction conditions and flavonoid content in different species of Potentillae was measured by using colorimetry. Under the conditions of 70 ℃, the extraction effects were the best. The crude flavonoid content was 17.98%. After being purified with AB-8 macroporous absorption resin, the content of flavonoid in crude flavonoid powder was up to 33.30%.

Potentilla chinensis SerFlavonoidsExtractionPurificationOrthogonal design

许正刚、闵运江

展开 >

安徽省淮南市淮南创大实业有限责任公司,安徽淮南,232087

皖西学院化生系,安徽六安,237012

委陵菜 黄酮 提取 纯化 正交设计

安徽省教育厅自然科学基金2010年度重点项目

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2010.38(6)
  • 9
  • 16