Optimization of Fermentation Conditions for Producing Xylanase and Identification of Hydrolysate of Xylan by the Xylanase from Streptomyces XP
[Objective]The study aimed to optimize the fermentation conditions for producing xylanase by Streptomyces XP,and identify the hydrolysate of xylan by the xylanase from this strain.[Method]The media composition,initial pH value of the media,fermentation temperature,fermentation time,liquid loaded volume for producing xylanase of this bacteria by using flask fermentation were optimized.Taking xylan from oats as substrate,the hydrolysate of the substrate by using crude enzyme was investigated by using TCL method.[Result]The best medium for producing xylanase from Streptomyces XP by using liquid fermentation was composed of 3.0% straw,0.2% NaOH,0.5% KH_2PO_4,0.5% (NH_4)_2SO_4. The initial pH value of the medium was 7.5,fermentation temperature was 37 ℃,fermentation time was 72 h,liquid volume was 60 ml in 250 ml triangle bottle,the rotating speed was 190 r/min.After xylan from oats was hydrolyzed by extracellular crude xylanase form Streptomyces XP, the major hydrolyastes of xylan by this enzyme displayed on TCL were xylotriose and xylibiose.And none xylose was found.[Conclusion]This study provided some technical reference for the research and development of producing xylanase strain.The hydrolyaste of xylan by this enzyme suggested that the enzyme had great value in the preparation of xylo-oligosaccharide.
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