首页|藏獒群体遗传变异的RAPD分析

藏獒群体遗传变异的RAPD分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]从分子水平上研究藏獒群体的遗传变异状况,为藏獒种质资源的保护提供依据.[方法]采用RAPD技术对不同地区的20份藏獒基因组DNA进行分析,并用群体遗传学软件PopGene32测定藏獒群体的有效等位基因数(Ne)、Nei氏平均预期基因杂合度(H)和Shannon遗传信息指数(I).[结果]共扩增出369条带,其中339条表现出多态性,多态性位点百分率为91.86%.PopGene32软件得到的藏獒群体的Ne、H和I值分别为1.751 2、0.416 2和0.602 1.[结论]藏獒群体内存在着丰富的遗传变异.
RAPD Analysis of Genetic Variation of Tibetan Mastiff
[ Objective ] To investigate the genetic variation of Tibetan mastiff at the molecular level and provide a basis for the protection of germplasm resources. [Methods] A total of 20 Tibetan mastiff blood samples in different regions were collected and the genomic DNA swere then analyzed using RAPD technique. The effective number of alleles (Ne) , Nei's average expected gene heterozygosity (H) and Shannon' s information index (Ⅰ) in Tibetan mastiff group were determined with population genetics software PopGene32. [ Results] A total of 369 bands were amplified from all the blood samples, in which as many as 339 bands showed polymorphism. The percentage of polymorphic loci was 91.86%. The values of Ne, H and I were 1.751 2, 0.416 2 and 0.602 1, respectively. [Conclusion] There is an abundance of genetic variations in the Tibetan mastiff group.

Tibetan mastiffRAPDGenetic variation

兰小平、郭宪、张敏、马刚、张海林、鄢珣

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天水师范学院生命科学与化学学院,甘肃天水,741000

中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730050

甘肃农业大学,甘肃兰州,730070

藏獒 RAPD 遗传变异

甘肃省科技攻关项目天水师范学院青年教师基金

GKC-97-27-5

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子：0.413
ISSN：0517-6611
年,卷(期)：2010.38(9)
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