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贺州大肉姜的组培快繁技术研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
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[目的]研究大肉姜组培快繁技术,为贺州大肉姜规模化生产提供技术支持.[方法] 以贺州大肉姜的芽作为外植体,研究不同消毒液和激素组合对外植体污染率、芽分化和生根的影响.[结果] 外植体用0.1% HgCl2消毒12 min效果最好,诱导芽分化的最佳培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA,最佳生根培养基为1/2MS+1.0 mg/L NAA,生根率可达100%.[结论] 该组培快繁技术可用于贺州大肉姜的规模化生产.
Tissue Culture and Rapid Propagation of Hezhou Ginger
[Objective] To study the tissue culture and rapid propagation technology of Hezhou ginger and provide technical support for large -scale production. [Method] Hezhou ginger (Zingiber officinale Rosc. ) buds were used as explants. The effects of different disinfectants and hor-monal combinations on contamination rate,bud differentiation and rooting were studied. [Result] The disinfection efficiency of explants were bet-ter with 0.1% HgCl2 for 12 min. The optimal medium for bud differentiation was MS+2.0 mg/ L6 -BA +0.1 mg/L NAA. The best medium for rooting was 1/2MS + 1.0 mg/L NAA,and the rooting rate was up to 100%. [Conclusion] The tissue culture and rapid propagation technology can be used for large-scale production of Hezhou ginger.

Hezhou gingerExplantTisssue culture

邓年方、潘百明、韦学丰

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贺州学院化学与生物工程系,广西贺州,542800

贺州大肉姜 外植体 组织培养

广西贺州市科技局科技攻关项目桂东特色资源重点建设实验室项目

0807003N2009SYS01

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子：0.413
ISSN：0517-6611
年,卷(期)：2010.38(11)
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