首页|野生与杂交天麻的DNA标记鉴定

野生与杂交天麻的DNA标记鉴定

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[目的]从分子水平鉴定野生和杂交天麻.[方法]合成SSR特异引物用于天麻基因组DNA的PCR扩增.[结果]SSR扩增重复性好、多态性高、分辨力强,野生和杂交天麻有各自特异的DNA标记.其中,杂交天麻均扩增出2条带,为杂合子;野生天麻只扩增出1条带,为纯合子.[结论]该研究为野生和杂交天麻种质资源的鉴别、保护、育种提供了一条有效的途径.
Identification of Wild and Cultivated Gastrodia elata Bl. by SSR Molecular Marker
[Objective] Wild and cultivated Gastrodia elata B1. were identified by SSR molecular marker. [ Method ] Distinctive primers were synthesized to the PCR amplification of the genomic DNA. [ Result ] The number of bands amplified was two in generative propagated individuals which was heterozygote and one in wild and clone propagated individuals which were homozygote. [ Conclusion ] SSR could effectively dis-tinguish heterozygote and homozygote, which would provide effective pathway for the identification and protection of Gastrodia elata B1, germ-plasm-resources and select breeding.

GermplasmsGastrodia elataSSRMarker of DNA

王晓丽、陈祖云、王晓玲、宋聚先

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贵阳医学院药用植物与生药学教研室,贵州贵阳,550004

贵州大学农学院,贵州贵阳,550025

种质 天麻 SSR DNA标记

科技部资助项目科技部资助项目科技部资助项目

2004BA721A33黔科合农社字20011125号黔科合中药专字[2003]51号

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2010.38(27)
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