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朱砂七多糖的抗氧化作用研究

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[目的]考察朱砂七多糖的抗氧化作用.[方法]分别以Fenton反应、邻苯三酚自氧化反应为产生羟自由基(·OH)和超氧阴离子自由基(O2-)模型,模拟人体胃液为亚硝化反应的环境,用分光光度法测试反应体系中活性氧和亚硝胺类的生成情况.[结果]朱砂七多糖浓度为5 mg/ml时,O2-和·OH清除率最高,分别为63%和48%,此浓度下其对亚硝酸铵合成的阻断率为41.27%;当朱砂七多糖浓度为0.09 mg/ml时,其对亚硝酸盐的清除率最高,为67.03%.[结论]朱砂七多糖具有较强的抗氧化活性.
Study on Antioxidative Activity of Polysaccharide in Polygonum cillinerve (Nakai) Ohwi
[ Objective ] The study was done to investigate the antioxidative activity of polysaccharide in Polygonum cillinerve ( Nakai) ohwi. [ Methods ] The hydroxyl radicals (· OH) and superoxide anion ( O2 - ) were produced by Fenton reaction and pyrogallolautoxidation system respectively. Sodium nitrite and nitrosamine were synthesized in simulated human gastric juice. Reactive oxygen species and nitrosamines were tested by UV spectrophotometer. [ Results]The scavenging rate to O2- and · OH were 63% and 48% when concentration of Polygonum cillinerve (Nakai) ohwi polysaccharide was 5 mg/ml, at which the blocking rate of nitrosamine was 41.27%. The scavenging rate to nitrite was 67.03% with Polysaccharide concentration of 0.09 mg/ml. [ Conclusion]Polygonum cillinerve (Nakai) ohwi polysaccharide is endowed with a strong anti-oxidation.

Polygonum cillinerve (Nakai) ohwi polysaccharideAntioxidantFree radicalNitrosamineNitrite

王晓梅、李宗孝、任莉君

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宝鸡文理学院化学化工系,陕西宝鸡,721013

陕西省植物化学重点实验室,陕西宝鸡,721013

朱砂七多糖 抗氧化 自由基 亚硝胺 亚硝酸盐

陕西省教育厅重点实验室基金资助项目宝鸡文理学院院级重点项目

08JZ08ZK0844

2010

安徽农业科学
安徽省农业科学院

安徽农业科学

北大核心
影响因子:0.413
ISSN:0517-6611
年,卷(期):2010.38(34)
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