Effect of Cryopreservation in Liquid Nitrogen on Germinability and Vigor of Wheat Seeds
[ Object ] The paper was to study the effect of cryopresorvation in liquid nitrogen on germinability and vigor of wheat seeds,in order to provide the theoretical basis for the cryopreservation of wheat germplasm resources. [ Method ] The seeds of four wheat varieties including Zhengmai 9023 ,Wanmai 48 ,Yangmai 158 and Annong 1032(waxy wheat)naturally dried after harvest were selected and sealed in plastic frozen pipes,then cryopreservated in liquid nitrogen for 7 d. Subsequently ,the seeds were taken out and thawed slowly in room temperature for 24 h. Seedling growth test ,cold soaking test, electrical conductivity test and simulated field germination test were carried out to determine the vitality indexes of wheat seeds. [ Result] After storage in liquid nitrogen for 7 d,the wheat seeds with the initial germination rate of 79.3％ -97.3％ still had the germination rate of 82.0％ -94.0％ ,79.3％ -98.0％ and 43.7％ -58.0％ in seedling growth test,cold soaking test and simulated field germination test,respectively. [ Conclusion]The experiment indicated that wheat seeds could be cryopreservated in liquid nitrogen.
国家科技期刊平台
NSTL
万方数据
维普
