首页|蕨菜中黄酮类化合物的提取及稳定性研究

蕨菜中黄酮类化合物的提取及稳定性研究

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[目的]提取、纯化蕨菜中黄酮类化合物,并对其稳定性进行研究.[方法]用浓度为70%的乙醇提取蕨菜中黄酮类化合物,用分光光度法测定黄酮的含量;并考察温度、光照、pH值、稳定剂、碳水化合物和金属离子对蕨菜黄酮稳定性的影响.[结果]蕨菜根中黄酮含量达4.2640%.蕨菜黄酮在pH值为4~6及有碳水化合物存在的条件下都能稳定存在,自然光和强光对黄酮含量有一定的影响,还原剂对黄酮含量有较大影响,Mg<'2+>、Al<'3+>、Fe<'3+>等金属离子对黄酮稳定性有影响.[结论]该研究可为蕨菜的进一步开发利用提供理论依据.
Study on the Extraction of Pteridium aquilinum var.latiusculum Flavonoid and Its Stability
[ Objective ] To extract and purify flavonoid from Pteridium aquilinum var. latiusculum, and to study its stability. [ Method ] Extracted with 70% alcohol,content of flavonoid from Pteridium aquilinum var. latiusculum was determined by UV spectrophotometry. Effect of temperature,illumination,pH value,stabilizing agent,carbohydrate and metal ions on the stability of flavonoid were investigated. [ Result ] The content of flavanoid in root of P. aquilinum var. latiusculum reached as high as 4. 264 0%. Except for carbohydrate and pH value in 4 -6,effects of other factors like natural light,highlight,reducing agent and Mg2+ ,Al3+ and Fe3+ on the stability of flavonoids from Pteridium aquilinum var. latitsculum were distinct. [ Conclusion ] This study provides theoretical evidence for the further application of Pteridium aquilinum var. latiusculum.

Pteridium aquilinum var.latiusculumFavonoidExtractionStability

陶永元、高顺玉、舒康云、徐成东

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楚雄师范学院化学与生命科学系,云南,楚雄,675000

滇中高原生物资源开发与利用研究所,云南,楚雄,675000

蕨菜 黄酮 提取 稳定性

国家自然科学基金

30760040

2011

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2011.39(7)
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