SPE Extraction of A.squarrosum Seed Oil and Analysis of Its Ingredients
[Objective]To optimize the extraction process of seed oil from Agriophyllum squarrosum in Xinjiang and analyze its ingredients. [ Method]The seed oil was extracted by SPE CO2 method, and extraction pressure, temperature and extraction duration were taken as investigated factors, and the optimum extraction conditions were obtained through orthogonal design on the basis of single factor test. Ingredients of A. Squarrosum seed oil were analyzed by GC-MS. [Result]The optimum extraction process was as follows; extraction pressure of 30 Mpa, extraction temperature of 40 ℃.extraction duration of 3 h. Under above conditions, the yield rate of A. Squarrosum seed oil reached as high as 13.62% , and the principle component of which was linoleic acid. [Conclusion] Iinoleic acid is one of the essential fatty acids that can reduce serum cholesterol and control arterialthrombus. SPE CO2 method is stable, efficient and of high application value.