首页|剑麻叶片内源激素高效液相色谱测定方法研究

剑麻叶片内源激素高效液相色谱测定方法研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]探讨剑麻叶片内源激素的高效液相色谱(HHC)测定方法.[方法]融合酶联免疫法( ELISA)的激素提取方法,对剑麻成熟叶片内源激素——赤霉素(GA3)、3-吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)进行了HPLC测定.[结果]该流程可联合测定GA3、IAA、ABA和ZT,回收率分别为78.81％ 、104.96％、81.24％和100.55％,且保留时间稳定,试验重现性好.[结论]整个提取、分离、分析流程较快速、准确,适合剑麻成熟叶片内源激素的分析测定.
High Performance Liquid Chromatography Determination of Endogenous Hormones in Mature Leaves of Agavybrid No.11648
[Objective] The aim was to study the high performance liquid chromatography (HPLC) determination of endogenous hormones in agavybrid No. 11648 leaves. [ Method ] Combining the hormone extraction methods of enzyme linked immunoassay, Gibberellin acid (GA3) ,3-indoleacetic acid (1AA), abscisic acid (ABA) and zeatin (ZT) in mature leaves of agavybrid No. 11648 were determined by HPLC [Result] The procedure could analyze the GA3 ,IAA, ABA and ZT in combination, and their recoveries were 78.81% , 104.96% , 81.24% and 100.55% respectively with stable retention time and good repeatability. [ Conclusion] The procedure is simple, rapid and accurate, so it is suitable for the efficient determination of endogenous hormone in mature leaves of agavybrid No. 11648.

High performance liquid chromatographyAgavybrid No. 11648Mature leavesEndogenous hormonesDetermination method

俞奔驰、黄富宇、吕平、梁立娟、彭静茹、张小玲

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广西壮族自治区木薯研究所,广西南宁53000

广西农垦山圩农场,广西扶绥532112

广西壮族自治区亚热带作物研究所,广西南宁530001

高效液相色谱 剑麻 成熟叶片 内源激素 测定方法

广西科学资金项目

桂科青0728048

2011

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子：0.413
ISSN：0517-6611
年,卷(期)：2011.39(22)
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