DNA Molecular Identification of Botanical Origin in Chinese Herb Qingjiao
[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation. [Methods] The cpDNA psbA-trnH and nrDNA ITS sequences of five botanical origin plants from Chinese herb Qingjiao, including Gentiana macrophylla Pall. ,C. straminea Maxim. ,G. crassicaulis Duthie ex Burk. , G. dahurica Fisch snd G. officinalis H. Smith, were amplified with PCR, and then sequenced by direct PCR sequencing method for homologous analysis. [ Results] The length of cpDNA psbA-tmH of five plants was 316 - 318 bp; there were seven different haplotypes and seven variable sites; the GC content of the sequence was 21.2% ; the phylogenetic clustering showed the same as haplotype analysis. The length of nrDNA ITS of five plants was 624 - 625 bp, there were five different hsplotypes and 13 variable sites; the GC conteni of the sequence was 59.3%. The result of phylogenelic clustering suggested that G. dahurica and G. straminea,G. macrophylla and G. officinalis clustered together as sister clade, respectively. [ Conclusion ] The nucleotide differences of nrDNA ITS regions could be used for distinguishing botanical origin in Chinese herb Qingjiao .
Chinese herb QingjiaopsbA-tmH sequencesITS sequencesPCRDNA molecular identification
国家科技期刊平台
NSTL
万方数据
维普
