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双角螺旋线虫扫描电镜观察

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[目的] 采用扫描电镜观察双角螺旋线虫(Helicotylenchus digoricus).[方法] 对采自浙江长兴的双角螺旋线虫进行了扫描电镜观察,对光镜下的形态结构进行了确认.[结果] 采用戊二醛—锇酸双固定及临界点干燥制备的线虫样本,虫体不皱缩,不变形,形态结构清晰,能够很好地进行扫描电镜观察.[结论] 为研究和防治双角螺旋线虫提供了参考.
Observation of Helicotylenchus digonicus by Scanning Electron Microscopy
[Objective] The paper was observe H. Digonicus by scanning electron microscopy. [ Method] H. Digonicus collected from Changxing of Zhejiang Province was observed under scanning electron microscopy, and its morphological structure was confirmed under optical microscopy. [ Result J The nemalode specimens Gxed by glutaraldehyde and osmium lelroxide and prepared by critical point drying were unmodified and unshrinkable with clear morphological structure .which could be scanned very well by SEM. [ Conclusion] The study provides reference for study and control of H. Digonicus.

H. DigonicusMorphological structureScanning electron microscopy

郑炜

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北仑出入境检验检疫局,浙江宁波 315800

双角螺旋线虫 形态特征 扫描电镜

2011

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2011.39(34)
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