[Objective] To review the research advances in DNA methylation of plants. [Method] The DNA methyltransferase and siRNA-guided DNA methylation process was reviewed and the relation between DNA methylation and other epigenetic modification was expounded. [ Result] DNA methylation played an important role in the epigenetic control system, it maintained the stability of genome and epigenetic modification in organic evolution process. siRNA played an irreplaceable role in RNA-mediated DNA methylation process, but the role of RdDM and methylation in gene regulation still needed further research. [ Conclusion ] The study gave a comprehensive summary of DNA methylation and its role in plant growth and response to adverse stress, which could enhance or inhibit epigenetic and endogenous gene silencing at the transcript level, so as to better improve the important transgenic crops.