首页|应用正交试验法优选茎瘤芥高效遗传转化体系

应用正交试验法优选茎瘤芥高效遗传转化体系

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[目的]建立茎瘤芥高效遗传转化体系,为茎瘤芥及芸薹属植物遗传转化奠定基础.[方法]应用正交试验法研究茎瘤芥遗传转化过程中预培养时间、共培养时间、侵染液浓度、侵染时间、对转化率的影响.[结果]最适合茎瘤芥CRY2基因RNAi载体遗传转化的条件为预培养3d、共培养4d、侵染液浓度OD600=0.5、侵染10 min,此时Kana选择压为30mg/L.[结论]优选出了农杆菌转化的最宜条件,遗传转化率有较大提高.
Optimization of Genetic Transformation System of Tumorous Stem Mastard by Orthogonal Design
[ Objective ] To establish the genetic transformation system, so us to lay foundation lor the genetic transformation of tumoroua stem mustard and other Brassica plants. [ Method] The effects of different time of precullure.cocullure,bacteria liquid concentration and dipping time on the transformation rate of lurnorous stem mustard were studied with orthogonal design. [ Result ] The best conditions for genetic transformation were 3 d preculture,4 d coculture, bacteria liquid concentration (OD600) 0.5 and 10 min dipping,then the Kan concentration was 30 mg/L. [Conclution] The optimum conditions for the transformation of tumorous stem mustard were selected, which greatly improved the transformation rale.

Tumorous Stem mustardOrthogonal designGenetic transformation

梁婷、刘毅、蔡应繁、岳显可、江怀仲、何晓红

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成都中医药大学研究生院,四川成都611130

重庆邮电大学生物信息学院,四川重庆400065

茎瘤芥 正变法 遗传转化

国家自然科学基金国家自然科学基金重庆市自然科学基金

3107146130771311cste2007BB1328

2012

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子：0.413
ISSN：0517-6611
年,卷(期)：2012.40(14)
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