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SSR方法标记桃果肉近核色素

Study on SSR Markers Linked to Flesh Color around the Stone of Prunus persica ( L.) Batsch

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[目的]利用SSR分子标记法标记桃(Prunus persica(L.)Batsch)果肉近核色素.[方法]以“重阳红”与“金保”2个桃品种为亲本构建正交F1群体,选取其中138株后代作为标记群体,采用分离群体分组分析(bulked segregant analysis,BSA)法,将果肉近核色素分为“有”和“无”2个基因池,应用SSR分子标记技术寻找与桃果肉近核色素性状基因连锁的分子标记.[结果]通过对256对引物的筛选,获得了3对与控制桃果肉近核色素性状基因连锁的分子标记,即UDP96-003、ch04g09和UDP97-402,同时计算得到这3个标记与桃果肉近核色素性状基因的遗传距离分别为16.7、10.1和17.0 cM.[结论]该研究为进一步筛选遗传距离更近的共显性分子标记奠定了基础.
[ Objective ] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica (L- ) Batsch. [ Method] P. persica ( L. ) Balsch varieties Chongyanghong and Jinhao were used as parents to construct F, orthogonal group. 138 F, individuals were selected as experimental materials for construction of color around the stone gene pool { B, ) and non-color around the stone gene pool ( Bz) by using hulked segrcgant analysis ( BSA) method, molecular markers linked to the flesh color around the stone of P. persica ( L. ) Bat so h were selected with SSR molecular marker technology. [ Result] After selection with 256 pairs of SSR primers, three pairs of mo lecular markers linked to the gene controlling flesh color around the stone of P. persica { L. ) Balsch were selected { UDP96-003 , ch04g09 and UDP97-402). In addition, genet it: distances of the three molecular markers with the gene controlling flesh color around the stone of P. persica (L. ) Batsch were calculated, which were 16.7, 10. 1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further se-lection of co-dominant molecular markers with closer genetic distance.

Prunus perstca {L. ) BatschFruit fleshFlesh Color around the stoneSSR

韩继成、刘国俭、常瑞峰、张新忠

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东北农业大学园艺学院,黑龙江哈尔滨150051

河北省农林科学院昌黎果树研究所,河北昌黎066600

果肉 近核色素 SSR

河北省农林科学院资助项目

A06120203

2012

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2012.40(16)
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