Effects of Toona sinensis Protein on the Antioxidant Capacity and Nonspecific Immunity of Mice
[ Objective] To study the effects of Toona sinensu protein on the antioxidant capacity and nonspecific immunity of mice. [ Method] Crude protein was extracted from T. sinensis by dialysis, freeze drying, and ammonium sulphate precipitation. Mice were given T. sinensis protein by intraperitoneal injection for 9 d in high-, middle- and low-dosages. Serums of half of mice were selected and their SOD and GSH-Px were detected by kits. Liver, spleen, and thymus were anatomized. MDA content and spleen and thymus indices were detected. The other half mice were given starch broth at 10 d by intraperitoneal injection. Then, peritoneal fluid was taken out and the phagocytic rate of macrophage was observed. [ Result] Content of T. sinensis crude protein was 84. 5% and the extraction rate was 5. 3%. T. sinensis protein could significantly enhance the activity of SOD in mice serum ( P < 0. 05 , high dosage P < 0. 01 ) . High-dosage T. sinensis protein could enhance the content of GSH-Px ( P < 0. 05) , spleen index ( P < 0. 05 , high dosage P < 0.01) , and phagocytic rate of macrophage ( P < 0. 05 ). [ Conclusion ] T. sinensis protein had certain in vivo antioxidanl capacity, and could promote the nonspecific immunity of mice.
Toona sinensis proteinMiceAntioxdation in vivoNonspecific immunity
国家科技期刊平台
NSTL
万方数据
维普
