首页|Folin-Ciocaileu 比色法测定新疆不同产地紫花苜蓿中总多酚含量

Folin-Ciocaileu 比色法测定新疆不同产地紫花苜蓿中总多酚含量

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  • 国家科技期刊平台
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[目的]测定新疆不同产地紫花苜蓿中总多酚的含量.[方法]以没食子酸作为对照品,以福林试剂-碳酸钠溶液体系为显色剂,采用Folin-Ciocaileu 比色法在检测波长760 nm处测定紫花苜蓿中总多酚含量.[结果]紫花苜蓿中总多酚含量的标准曲线的回归方程为Y=91x +0.011 8(r =0.999 5),表明在0～6 mg/ ml浓度范围内浓度与吸光度的线性关系良好,平均回收率分别为102.34％、103.94％、102.81％.[结论]该方法简便、快速、准确,是检测新疆不同产地紫花苜蓿总多酚成分的可靠方法.
Determination of the Content of Total Polyphenol for Medicago sativa L.of Xinjiang by Folin-Ciocaileu
[Objective] To establish an UV method for determination of the content of total polyphenol for M. sativa of Xinjiang. [ Method] With gallic acid as CK, Folin-Ciocaileu was adopted determining the content of total polyphenol by UV at 760 nm. [ Results] The regressive equation of standard curve is Y= 91x + 0. 01l8 (r=0.9995 ). It was indicated that in the range of 0- 6 mg/ml, the liner relationship between concentration and absorbance is good. The average recovery was 102.34% , 103.94% , 102.81%. [Conclusion] The method is simple, rapid and accurate, which is a reliable method for detecting total polyphenol from M. sativa in Xinjiang.

Medicago sativa of XinjiangTotal polyphenolFolin-Ciocaileu colorimetry

燕雪花、刘宏炳、孙芸、田树革、雷海民

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新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830011

新疆医科大学中医学院,新疆乌鲁木齐830011

北京中医药大学中药学院,北京 100029

新疆紫花苜蓿 总多酚 Folin-Ciocaileu 比色法

新疆医科大学科研创新基金新疆名医名方与特色方剂学实验室开放项目

XJC201003XJDX0910-2010-07

2012

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子：0.413
ISSN：0517-6611
年,卷(期)：2012.40(32)
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