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矮牵牛愈伤组织耐盐性研究

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[目的]了解矮牵牛愈伤组织的耐盐能力.[方法]将矮牵牛嫩叶接种到8种不同盐浓度的MS培养基中培养,观察愈伤组织生长分化情况.[结果]当培养基盐度为0.4%时,矮牵牛愈伤组织诱导率与对照无差异;当培养基盐度为0.8%以下时,提高盐度能促进矮牵牛愈伤组织的芽分化率;当培养基盐度大于0.8%时,矮牵牛愈伤组织的生长分化受到抑制;当培养基盐度达到2.8%时,矮牵牛外植体全部死亡.[结论]矮牵牛愈伤组织可适应0.8%以下的盐度.
Study on Salt-tolerance of Petunia hybrida Callus
[ Objective ] To study the salt-tolerance ability of Petunia hybrida callus. [ Method ] The callus growth differentiation was observed by inoculating P. hybrida leaves into eight different salt concentrations MS medium for culture. [Result] In0.4% salinity medium, P. hybrida callus induction rate is the same with control group; below 0. 8% , improving salinity could promote bud differentiation rate of P. hybrida callus; a-bove 0.8% , the growth differentiation could be inhibited;in the medium salinity reached 2.8% , P. hybrida explant all died. [Conclusion] P. hybrida callus can adapt to the salinity below 0. 8%.

Petunia hybrida VilmCallusSalt-tolerance

杨晓玲、郭金耀

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淮海工学院海洋学院,江苏连云港222005

矮牵牛 愈伤组织 耐盐性

中央财政支持地方高校发展专项资金资助

CXTD34

2013

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2013.(2)
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