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独花兰组织培养研究

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[目的]研究独兰花的组织培养方法.[方法]以独兰花假鳞茎为外植体,以1/2MS +0.5 mg/L 6-BA +0.05 mg/L NAA为芽诱导培养基,以1/2MS+ 1.5 mg/L 6-BA+ 0.05 mg/L NAA为生根培养基,研究外植体的芽诱导和生根情况.[结果]外植体消毒的最佳方式为75%酒精30 s+0.2% HgCl 6~7 min;试验外植体的污染率、诱导率、死亡率、成活率、生根率分别为14.3%、57.1%、28.6%、50.0%、33.9%.[结论]该研究为独花兰的规模化生产提供了技术指导.
Study on Tissue Culture of Changnienia amoena
[Objective] The aim was to study the tissue culture method of Changnienia amoena.[Method] Using the pseudobulb of C.amoena as explant,the 1/2MS + 0.5 mg/L 6-BA + 0.05 mg/L NAA as bud induction medium and the 1/2MS + 1.5 mg/L 6-BA + 0.05 mg/L NAA as rooting medium,the conditions of bud induction and rooting of the explants were studied.[Result] The best sterilized method of the explants was 75% alcohol for 30 s and 0.2% HgCl 6-7 min; the contamination rate,induction rate,mortality rate,survival rate and rooting rate of the explants were 14.3%,57.1%,28.6%,50%,33.9% respectively.[Conclusion] The study provided technical guidance for the large-scale production of C.amoena.

Changnienia amoenaTissue culturePseudobulbRapid propagation

刘国顺、杨丽、董卉卉、哈登龙、琚煜熙、王晶、马冠男

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信阳市林业科学研究所,河南信阳464031

鸡公山国家级自然保护区管理局,河南信阳464133

信阳农林学院,河南信阳464000

独兰花 组织培养 假鳞茎 快速繁殖

信阳市科技计划项目

KJGG1110

2013

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2013.(22)
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