[Objective] The aim was to study the tissue culture method of Changnienia amoena.[Method] Using the pseudobulb of C.amoena as explant,the 1/2MS + 0.5 mg/L 6-BA + 0.05 mg/L NAA as bud induction medium and the 1/2MS + 1.5 mg/L 6-BA + 0.05 mg/L NAA as rooting medium,the conditions of bud induction and rooting of the explants were studied.[Result] The best sterilized method of the explants was 75% alcohol for 30 s and 0.2% HgCl 6-7 min; the contamination rate,induction rate,mortality rate,survival rate and rooting rate of the explants were 14.3%,57.1%,28.6%,50%,33.9% respectively.[Conclusion] The study provided technical guidance for the large-scale production of C.amoena.