Advances in Tissue Culture and Rapid Propagation of Dendrobium candidum Wall.ex Lindl.
D.candium Wall.ex Lindl.is a traditional Chinese medicinal herb with low natural propagation and wild resource scarcity,the research of D.candium rapid propagation has made great progress in recent years.In this paper,the researches of tissue culture and rapid propagation of D.candidum Wall.ex Lindl.were reviewed from the aspects of selection of explants in tissue culture,protocorm multiplication and differentiation,rooting and strengthening seedling and transplanting.The deficiencies of current researches were proposed,i.e.long cultivation circle of seedlings,high cost; poor researches on transplanting techniques,low survival rate of transplanted seedlings; limited researches on accumulation of active ingredients in seedling cultivation.In view of the problems,the future research directions were discussed,i.e.researches on illumination,temperature and humidity,multiplication and differentiation of protocorm,shortening seedling cultivation circle,improving production efficiency; devoting more in fundamental researches on transplanting techniques,improving transplanting rate; enhancing researches on the relationship between accumulation of active ingredients and cultivation conditions in tissue culture,improving concentration of active ingredients of seedling.The research provides references for the large-scale production of D.candidum Wall.ex Lindl..