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天胡荽抗肿瘤活性成分研究

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[目的]研究天胡荽的抗肿瘤活性成分.[方法]利用色谱手段进行分离纯化,应用NMR方法鉴定结构,并进行MTT法抗肿瘤试验.[结果]从天胡荽中分离鉴定了4种化合物(化合物1~4).天胡荽的各萃取部位对LA795细胞株有一定的抑制作用,其中以正丁醇层萃取物在高浓度800雌/ml时的抑制作用较强,而石油醚和乙酸乙酯萃取物的抑制作用相对较弱.单体化合物对LA795细胞株的抑制作用均较弱.[结论]4个化合物有一定抑制肿瘤细胞生长作用.
Study on Anti-tumor Constituents from Hydrocotyle sibthorpioides
[Objective] To study anti-tumor constituents from Hydrocotyle sibthorpioides.[Method] The constituents were isolated by chromatography and identified by NMR spectroscopic methods.The anti-tumor experiment in vitro,the MTT was used to screen constituents of Hydrocotyle sibthorpioides.[Result] Compounds 1-4 were isolated and identified.Each extracted parts of Hydrocotyle sibthorpioides all have a certain inhibition effect on LA795,the inhibition effect of N-butanol extract in high concentration of 800μg/ml is the strongest,while the inhibition effects of petroleum ether and ethyl acetate extracts are relative weaker.[Conclusion] The 4 compounds all have a certain inhibition effect on the growth of tumor cells.

Hydrocotyle sibthorpioidesChromatographyAnti-tumor

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浙江海洋学院萧山科技学院,浙江杭州311231

天胡荽 色谱 抗肿瘤

浙江省教育厅项目

Y200908478

2014

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2014.(11)
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