[Objective] To observe the inhibitory effects of Rheum officinale and Flos caryophylli on Vibrio harveyi and its biofilm.[Method] The inhibitory effects of Rheum offtcinale and Flos caryophylli on Vibrio harveyi were tested by disc agar diffusion,and the MIC and MBC were detected by the double dilution ; the effects of Rheum officinale and Flos caryophylli on the formation of Vibrio harveyi biofilm were measured by MTT assay.[Result] Rheum officinale had inhibitory effects with the diameter of inhibition zone (DIZ) of (15.31 ± 0.4) mm,and the MIC and MBC were 7.183 mg/ml; Flos caryophylli had inhibition effects with DIZ of (11.53 ±0.6) mm,and the MIC and MBC were 15.625 mg/ml,62.5 mg/ml.Both Rheum officinale and Flos caryophylli exhibited a potent inhibitory activity against Vibrio harveyi biofilm formation (P ＜ 0.01).[Conclusion] Rheum officinale and Flos caryophylli could significantly inhibit Vibrio harveyi and its biofilm.
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维普
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