Measure of Total Polyphenols Content in Zhaotong Grape and Grape Wine by Folin-Ciocalteu Method
[Objective] In order to provide scientific basis for production and brewing of Vitis vinifera in Zhaotong,the total polyphenol content in grapes was measured by Folin-Ciocalteu method.[Method] Using methyl alcohol as the solvent,total polyphenols of Vitis vinifera was extracted by supersonic method,then added Folin-Ciocalteu reagent and 50 g/L Na2CO3 solution,and measured the absorbancy at 765 nm.[Result] Basing on gallic acid,the mass concentration had an obvious linear relation (r =0.999 5) with absorbancy within the scope of 1-40 mg/L.The parallel determination (n =6) of total polyphenol results were that,when RSD was between 1.1％ to 1.6％,the adding standard recovery of Vitis vinifera and grapes wine were 98.8％,98.3％,RSD 1.8％,2.0％.[Conclusion] This method is convenient,repeatable and faithful,which can be used for determination of polyphenol in grapes and grape wine.
国家科技期刊平台
NSTL
万方数据
维普
