Study of Solid Substrate Fermentation with Complex Microbial on Rapeseed Meal
[Objective] To study effects of compound microbial solid fermentation on detoxification and nutritional value of rapeseed meal.[Method] With common rapeseed meal as raw material,using modem solid fermentation principle,Lactobacillus plantarum and Aspergillus niger were adopted to conduct solid fermentation.The optimal fermentation technique of degradating anti nutritional factors and improving crude protein content was discussed.[Result] The results showed that complex microbial solid fermentation can significantly reduce content of anti nutritional factors in rapeseed meal.The fermentation effect of complex bacterias is superior than single bacteria.The ratio between Lactobacillus plantarum and Aspergillus niger is 3∶1,innoculation amount 20%,solid-liqud ratio 1∶0.8 g/ml,rapeseed meal:maize:bran =8∶ 1∶ 1,fermentation time 48 h.After fermentation,crude protein content increased 6.57%,small peptide increased 88.76%,crude fiber content reduced 11.31% ; Isothiocyanate,Oxazolidine thione,Glucosinolate reduced 64.00%,81.82%,75.16%,respectively; total amino acid content increased 11.59%.[Conclusion] The study can provide an effective way for development and utilization of rapeseed meal in biological feed.