Study on the Sperm Sex-sorting of Working Dogs by Flow Cytometry
[Objective] The research aimed to explore the sex-sorting method of canine spermatozoa by using flow cytometry.[Method] The semen samples were collected from Kunming dogs and German shepherd dogs and stained with different concentrations (6,8,10 and 12 μl/ml) of Hoechst 33342.The influences of different concentrations of Hoechst 33342 and different dog individuals on the sex-sorting efficiency were discussed.[Result] When the concentrations of Hoechst 33342 was 6,8,10 μl/ml,fresh canine sperm could not be effectively separated.When the concentration of Hoechst 33342 was 12 μl/ml,fresh canine sperm could be effectively and stably separated.The death rate of sperm in fresh semen of different male dogs showed an obvious negative correlation with the sex-sorting speed,but the sex-sorting accuracy had no obvious correlation with the death rate of sperm in fresh semen.[Conclusion] When the concentration of Hoechst 33342 was 12 μl/ml,the sex-sorting of canine spermatozoa could be successfully performed by using flow cytometry.It was of great significance for improving the sex-sorting efficiency of sperm and the quality of separated sperm to select suitable male dog.
国家科技期刊平台
NSTL
万方数据
维普
