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聚乙二醇对低温胁迫下玉米幼苗生理生化指标的影响

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[目的]在实验室条件下,研究聚乙二醇(PEG)对低温胁迫下玉米幼苗生理生化指标的缓解效应.[方法]在28℃条件下盆栽玉米,待玉米长到三叶期,对玉米幼苗进行8℃、3d的低温胁迫处理,同时施用浓度30% PEG喷洒叶片,3d后测定玉米幼苗生理生化指标的变化.[结果]受低温胁迫后玉米幼苗丙二醛、可溶性糖含量及过氧化物酶活性均显著升高(P<0.05),叶绿素含量显著降低(P<0.05);对低温胁迫下的幼苗施用浓度30% PEG后,丙二醛、可溶性糖含量以及过氧化物酶活性较低温对照组显著降低(P<0.05),叶绿素含量显著升高(P<0.05),施用浓度30% PEG后各指标均达到或超过正常对照组水平.[结论]浓度30% PEG对低温胁迫下玉米幼苗具有缓解效果.
The Effects of Polyethylene Glycol on Physiological and Biochemical Characteristics of Corn Seedlings under Low Temperature Stress
[Objective] To investigate the mitigative effect of polyethylene glycol (PEG) on physiological and biochemical characteristics of corn seedlings under low temperature stress in lab.[Method] Firstly,the corns were potted at 28 ℃.At three-leaf stage,the corn seedlings were stressed by 8 ℃ low temperature for three days.At the same time,the leaf was sprayed by PEG (30%).After three days,the physiological and biochemical characteristics of corn seedlings were determined.[Result] When core seedlings were stressed by low temperature,the contents of malondialdehyde (MDA) and soluble sugar increased significantly (P < 0.05),and the peroxidase (POD) activity also increased (P < 0.05),but the content of chlorophyll decreased dramatically (P < 0.05).After the above stressed corn seedlings were sprayed by PEG (30%),MDA content,soluble sugar content and POD activity decreased (P <0.05),and the chlorophyll content increased (P <0.05) comparing with low temperature control.All levels using PEG (30%) can reach or exceed the low temperature control.[Conclusion]PEG (30%) has mitigative effect on corn seedlings under low temperature stress.

Low temperature stressPolyethylene glycolCorn seedlingPhysiological and biochemical characteristicsMitigative effect

朴樱姬、姜宏伟、于洋、刘若岑、杨艳波、武燕

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大庆师范学院,黑龙江大庆163712

低温胁迫 聚乙二醇 玉米幼苗 生理生化指标 缓解效果

黑龙江省大学生创新创业训练计划项目

201410235180

2015

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2015.(14)
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