首页|壳寡糖抗氧化性及对鲫鱼保鲜性能的研究

壳寡糖抗氧化性及对鲫鱼保鲜性能的研究

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[目的]研究壳寡糖的抗氧化性及壳寡糖对冷藏鲫鱼保鲜性能的影响.[方法]以壳寡糖为原料,采用邻二氮菲-Fe2氧化法、DP-PH法等对壳寡糖的抗氧化性进行了测定,以pH、TVB-N及TBA为指标研究了壳寡糖对鲫鱼保鲜性能的影响.[结果]研究表明,1.5mg/ml壳寡糖溶液的还原能力相当于50 μg/ml阳性对照VC的还原能力;1 mg/ml壳寡糖溶液对羟自由基的清除率为45%,相当于100μg/ml阳性对照VC对羟自由基的清除率;4 mg/ml的壳寡糖溶液对DPPH自由基的清除率达到97.8%.新鲜鲫鱼在4℃冰箱冷藏条件下,壳寡糖溶液涂膜处理组和对照组在第8天时的TVB-N值分别为252.0和196.0 mg/kg;pH分别为7.73和6.69;TBA值分别为1.552和0.670.综合各项指标的变化,壳寡糖涂膜保鲜的鲫鱼货架期明显比对照组长,表明壳寡糖具有较好的抗氧化能力和保鲜性能.[结论]研究可为壳寡糖在更多领域更广泛的开发应用奠定基础.
Research of Oligo-chitosan's Antioxidant and Preservation Properties of Carp
[Objective] To study the effects of oligo-chitosan' s antioxidant and its impact on the preservation properties of the fresh frozen carp.[Method] With the laboratory-made oligo-chitosan as raw materials,antioxidant activity were measured using phenanthroline-Fe2+ oxidation method and DPPH method,by determining changes of pH,TVB-N and TBA,the oligo-chitosan' s preservation properties on carp were studied.[Result] The results showed that 1.5 mg/ml of oligo-chitosan' s reducing power equivalent to 50 μg/ml positive control Vc reducing capacity;At a concentrate of 1 mg/ml,the scavenging of COS quaternary ammonium towards hydroxyl radicals is 45%,equivalent to 100 μg/ml positive control for hydroxyl radical scavenging rate.At a concentrate of 4 mg/ml,the scavenging of COS quaternary ammonium towards DPPH is 97.8%.Under the condition of 4 ℃ refrigerator refrigeration,TVB-N value of oligo-chitosan group and control group are 252.0 and 196.0 mg/kg at the 8th day;pH value are 7.73 and 6.69;TBA are 1.552 and 0.670,respectively.The shelf-life of carp was extended for about 3 days,result shows that oligo-chitosan has good resistance to oxidation capacity and preservation properties.[Conclusion] The study can lay a foundation for extensive development of oligo-chitosan in more fields.

Oligo-chitosanAntioxidantCarpPreservation properties

刘敏、包静、刘均忠、孙谧

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大连海洋大学食品科学与工程学院,辽宁大连116023

中国水产科学研究院黄海水产研究所,山东青岛266071

壳寡糖 抗氧化性 鲫鱼 保鲜性能

国家“863”计划资助项目国际科技合作与交流专项

2011AA090703号2014DFG30890号

2015

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2015.(20)
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