Effect of PEG Stress on Germination Characteristics and Chromosome Control in Wheat Substitutions Lines
[Objective] To clear effect of PEG stress on germination characteristics and chromosome control in wheat substitutions lines and locate chromosome of related genes so as to provide theoretical basis for select drought-resistance high yield lines.[Method] We used Chinese Spring(CS)-Synthetic 6x substitutions lines and parents cultivars "Chinese Spring" and Synthetic 6x as materials to study the effect on seed germination characteristics and chromosomal effect by measuring germination percentage (GP),germinating energy (GE) and germination index (GI) under demonized water (control) and Polyethylene glycol (PEG)-6000 employed as osmotic for simulating water stress.[Result] The GP,GE and GI of CS-Synthetic 6x substitutions lines and parents decreased compared to the control conditions under the PEG waterstress.GP,GE and GI of 5B,6B,4D,6D and 7D substitutions lines were significantly higher than those of CS.[Conclusion] The genes of stimulating germination may be located on 5B,6B,4D,6D and 7D Chromosomes of Synthetic 6x,and they can be used in breeding for drought resistance.
Wheat substitutions linesPEG stressGerminating energyGermination percentageGermination index