油棕仁贮藏蛋白质亚基组成和含量分析
Study on Subunits of Oil Palm Kernel Storage Proteins
张璐 1郑亚军 2李艳 3张有林 3张润光 3张玉峰4
作者信息
- 1. 陕西师范大学食品工程与营养科学学院,陕西西安710119;海南大学园艺园林学院,海南海口570228
- 2. 陕西师范大学食品工程与营养科学学院,陕西西安710119;中国热带农业科学院椰子研究所,海南文昌571339
- 3. 陕西师范大学食品工程与营养科学学院,陕西西安710119
- 4. 中国热带农业科学院椰子研究所,海南文昌571339
- 折叠
摘要
[目的]为油棕仁中蛋白质的进一步研究与开发利用提供指导.[方法]采用非变性聚丙稀酰胺凝胶电泳(Native-PAGE)和十二烷基硫酸钠-聚丙稀酰胺凝胶电泳(SDS-PAGE)对油棕仁中贮藏蛋白质亚基进行研究,分析油棕仁蛋白主要亚基的组成、分子量、含量、二硫键的位置及种间差异.[结果]油棕仁中贮藏蛋白质在浓缩胶浓度为7.5％、分离胶浓度为12％的SDS-PAGE凝胶系统中可以得到很好的分离.在油棕仁贮藏蛋白中含有6条主要亚基,分子量范围为18.8 ～51.5 kDa,主要是中、小分子量亚基;各亚基之间含一定量的二硫键.非变性凝胶电泳分析表明,油棕仁贮藏蛋白中含有4个不同分子量、不同电荷的组分.[结论]该研究所选的不同油棕品种之间亚基的组成和含量没有显著性差异.
Abstract
[Objective] The research aimed to provide the guidance for the further study and the development and utilization of protein in oil palm kernel.[Method] The subunits of storage proteins in oil palm kernel were studied by SDS-PAGE and Native-PAGE,including their content,molecular weight,content of disulfide bonds and variance difference.[Result] Storage proteins in oil palm kernel were well separated by SDS-PAGE with 12％ separation gel and 7.5 ％ pile gel.Under this condition,the storage proteins in oil palm kernel were separated into 6 main subunits,with a molecular weight range of 18.8 ～51.5 kDa.This meant that the main subunits were lower molecular weight in oil palm kernel storage proteins.Compared with the result of SDS-PAGE with Native-PAGE,there were some disulfide bonds among the subunits of oil palm kernel storage proteins.There were four peptides with different molecular and different electric charge separated by Native-PAGE.[Conclusion] There was no significant difference between the two kinds of oil palm.
关键词
油棕仁/贮藏蛋白/亚基/SDS-PAGEKey words
Oil palm kernel/Storage protein/Subunits/SDS-PAGE引用本文复制引用
基金项目
国家科技支撑项目(2012BAD31B03)
海南省重大科技项目(ZDZX2013011)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据