患兔流行性腹胀病家兔肠道菌群结构的ERIC-PCR指纹图谱分析
ERIC-PCR Fingerprint of Intestinal Microflora of Epidemic Abdominal Distention Disease in Domestic Rabbit
胡波 1范志宇 1王芳 1魏后军 1宋艳华 1仇汝龙 1刘星 1徐为中 1薛家宾1
作者信息
- 1. 江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏南京210014
- 折叠
摘要
[目的]建立患兔流行性腹胀病家兔肠道细菌的DNA指纹图谱,并分析其肠道菌群结构特征的整体差异.[方法]提取流行性腹胀病兔及健康兔肠道内容物细菌总DNA,应用肠杆菌基因间重复共有序列基因扩增技术(ERIC-PCR)建立肠道菌群的DNA指纹图谱,并分析其整体差异.[结果]病兔大肠样本DNA条带明显少于健康兔对照,而小肠样本在二者间无明显差异,说明病兔与健康兔大肠肠道菌群存在整体差异.病兔大肠样本DNA在约350 bp处出现1条主带,同时伴有若干较弱的带,而健康兔对照大肠样本DNA条带均无明显规律.病兔大肠肠道的微生物群落多样性指数为(2.03±0.49),健康兔大肠肠道的微生物群落多样性指数为(3.30±0.31),差异极显著(P＜0.01).[结论]兔流行性腹胀病发病兔大肠中肠道菌群结构多样性降低,可能存在单一或几种特定的肠道优势菌群.
Abstract
[Objective] To establish the DNA fingerprint of intestinal microflora of epidemic abdominal distention disease in domestic rabbit,and to analyze the overall structural differences of intestinal microflora.[Method] The total DNAs were extracted from the intestinal samples of rapids,and enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction (ERIC-PCR) was used to set up the DNA fingerprint of intestinal microflora.The overall differences existed among the fingerprints of intestinal microflora were compared.[Result] The number of DNA bands about large intestinal samples was obviously less in diseased rabbits than in healthy subjects,while the small intestinal samples showed similar DNA bands,indicating that significant differences existed in large intestine between diseased rabbits and healthy ones.The principal band of DNA fingerprint of READ samples appeared at about 350 bp,while no principal band was found in healthy samples.The corresponding Shannon' s diversity indexes of diseased rabbits and healthy rabbits were (2.03 + 0.49) and (3.30 ± 0.31),respectively,showing significant differences (P ＜ 0.01).[Conclusion] The diversity of large intestine microflora signiticantly reduces compared with healthy rabbits.It is likely that one or more principal microflora exist in the intestinal tissue of READ rabbits.
关键词
兔流行性腹胀病/ERIC-PCR/肠道菌群/多样性指数Key words
Rabbit epidemic abdominal distention disease/ERIC-PCR/Intestinal microflora/Diversity index引用本文复制引用
基金项目
现代农业产业技术体系建设专项(CARS-44)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据