疏花蔷薇果总多酚抗氧化活性
Antioxidant Activity of Total Polyphenols from Rosa laxa Ratz.Fruits
王东东 1汤紫薇 1党稳梅 1郭萍萍 1王慧杰 1田莉1
作者信息
- 1. 新疆医科大学中医学院,新疆乌鲁木齐830011
- 折叠
摘要
[目的]研究疏花蔷薇果中总多酚的抗氧化能力,为进一步开发疏花蔷薇果提供试验数据.[方法]利用已优化的响应曲面法提取疏花蔷薇果中的总多酚,并测定其含量;应用3种抗氧化模型(清除DPPH自由基、清除羟基自由基、清除超氧阴离子自由基),建立疏花蔷薇果中的多酚类成分抗氧化能力模型,以抗坏血酸为阳性对照,评价疏花蔷薇果中总多酚的体外抗氧化能力.[结果]在试验浓度范围内,疏花蔷薇果总多酚对羟基自由基、超氧阴离子自由基、DPPH自由基均有较强的清除能力,其清除率与总多酚浓度有一定的量效关系.[结论]疏花蔷薇果总多酚有明显的清除自由基能力,可以作为一种有效的天然抗氧化剂来开发利用.
Abstract
[Objective] To study antioxidant activity of total polyphenols from Rosa laxa Ratz.fruits,to provide experimental data for the further development of Rosa laxa Ratz.fruits.[Method] Total polyphenols from Rosa laxa Ratz.fruits was extracted by optimized response surface methodology,and its content was determined.Utilizing the three types of antioxidant activity models(the active effect of clearing DPPH radical,hydroxyl radical and superoxide anion radical),the antioxidant activity models of polyphenols ingredient from Rosa laxa Ratz.fruits were established,with ascorbic acid as a positive control,the antioxidant activity ability in vitro of total polyphenol from Rosa laxa Ratz.fruits was evaluated.[Result] With a certain range of concentrations,total polyphenols from Rosa laxa Ratz.fruits showed strong scavenging capacities on the DPPH radical,hydroxyl radical and superoxide anion radical.And the effect was enhanced with an increase in the concentration,and the scavenging rate was certain dose-dependent manner.[Conclusion] Total polyphenols from Rosa laxa Ratz.fruits has significant capacity of scavenging free radical,it can be applied as an effective natural antioxidant.
关键词
疏花蔷薇果/总多酚/抗氧化活性Key words
Rosa laxa Ratz.fruits/Total polyphenols/Antioxidant activity引用本文复制引用
基金项目
国家级大学生创新项目(CX2014016)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据