不同提取方法对水龟虫多糖含量的影响
Effect of Different Extraction Methods on the Polysaccharide Content in Hydrophilus acuminatus
许龙 1常江春 2高敏 2房月春 2栾雪 2陈琦 2罗志文1
作者信息
- 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007;佳木斯大学应用昆虫研究所,黑龙江佳木斯154007
- 2. 佳木斯大学生命科学学院,黑龙江佳木斯154007
- 折叠
摘要
[目的]研究水龟虫多糖的提取条件.[方法]分别采用3种方法对水龟虫多糖进行了提取,采用苯酚-硫酸法进行多糖含量测定,对比不同提取方法中多糖的含量差异.[结果]热水浸提、碱提法和酶解法3种方法对水龟虫多糖提取所得多糖的含量分别为1.53％、2.61％、2.66％.[结论]通过试验确定了酶解法为水龟虫多糖的提取最佳方法.
Abstract
[Objective] To study conditions for extracting polysaccharide from Hydrophilus acuminatus.[Method] Three methods were adopted to extract polysaccharide from Hydrophilus acuminatus,polysaccharide content was determined by phenol sulfuric acid method,and polysaccharide contents extracted by different methods were compared.[Result] The polysaccharide content in Hydrophilus acuminatus extracted by hot water extraction,alkali extraction and enzymatic hydrolysis was 1.53％,2.61％,2.66％,respectively.[Conclusion] Enzymatic hydrolysis is the best method for extracting polysaccharide from Hydrophilus acuminatus.
关键词
水龟虫多糖/提取方法/含量测定/条件因素Key words
Hydrophilus acuminatus polysaccharide/Extraction method/Determination of content/Condition factors引用本文复制引用
基金项目
黑龙江省大学生科技创新创业训练计划项目校级项目(2014xj016)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据