亚硝基胍诱变丙酸杆菌选育高产抑菌物质菌株
Screening of Propionibacterium shermanii Strains with High Production Antibacterial Metabolites by NTG Mutation
何新舟 1查东风 2黄汉峰 2杨雪霞 3常忠义 1高红亮1
作者信息
- 1. 华东师范大学生命科学学院,上海200241
- 2. 河南省商丘市农业局经作中心,河南商丘476000
- 3. 东华大学化学化工与生物工程学院,上海201620
- 折叠
摘要
[目的]提高丙酸杆菌代谢物的抑菌活性,为薛氏丙酸杆菌代谢物的工业化生产奠定基础.[方法]以实验室保藏的薛氏丙酸杆菌H1310为出发菌株,采用亚硝基胍(NTG)进行诱变处理,待突变菌株长出后采用双层平板筛选法进行筛选,而后通过深层液体发酵对筛选到的菌株进行复筛.[结果]当最优的NTG诱变浓度为0.3 mg/mL时,致死率为90.69％.采用双层平板法,从3 564株菌落中挑选出87株突变株,进一步进行复筛,获得一株突变菌株,其代谢物抑菌活性达(28.30±2.47)AU/mL,比出发菌株提高43.87％.[结论]确定了丙酸杆菌NTG诱变选育的试验方法,获得一株抑菌活性高的遗传性能稳定的菌株.
Abstract
[Objective] To improve the antibacterial activity of the metabolite of Propionibacterium shermanii and lay a foundation for the industrial production of the metabolite.[Method] This research used Propionibacterium shermanii H1310 as the original strain,through nitrosoguanidine (NTG) mutagenesis,using the double-layer plate screening method to screen the mutant strains.The strains which were surrounded by clear inhibition zone were cultivated and measured the antibacterial activity.[Result] The optimal concentration of NTG was 0.3 mg/mL,for the lethality rate was 90.69％.87 strains chosen from 3 564 strains were fermented again.A high antibacterial metabolites stain NTGll0605 was selected and its antibacterial activity reached (28.30 ± 2.47) AU/ mL,which was 43.87％ higher than the original strain.[Conclusion] The experimental methods of mutation breeding of Propionibacterium shermanii is confirmed and a strain which produced more antibacterial substances is obtained.
关键词
亚硝基胍诱变/薛氏丙酸杆菌/双层平板筛选法/抑菌活性Key words
Nitrosoguanidine mutation/Propionibacterium shermanii/Double-layers plate screening method/Antibacterial activity引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据