改进的双波长法测定二元混合体系中葡萄糖和木糖含量
Detection of Glucose and Xylose in Duplex Mixture by Improved Dual-wavelength Spectrophotometry
朱捷 1葛奉娟 1王欲晓1
作者信息
- 1. 徐州工程学院化学化工学院,江苏徐州221111
- 折叠
摘要
[目的]采用改进的双波长法测定混合糖液中葡萄糖和木糖含量.[方法]采用分光光度法(Douglas法)对标准糖溶液的糖含量进行了测定,并对传统的双波长法的波长选择和标准曲线拟合进行了改进:通过误差分析确定测定波长,对木糖显色反应在550nm处的标准曲线进行二次方程拟合,使其更适于低浓度木糖的检测.[结果]该方法可同时测定混合体系中葡萄糖和木糖的含量,总糖、木糖和葡萄糖测定的回收率均为94.00％～101.60％,准确度较高.[结论]该方法能满足植物纤维水解液中糖含量的常规分析要求.
Abstract
[Objective] To detect the accharide contents of glucose and xylose in duplex mixture by improved dual-wavelength spectrophotometry.[Method] Saccharide content in standard solution was detected by spectrophotometry (Douglas method).The wavelength selection and standard curve fitting were improved in traditional dual-wavelength method : Detection wavelength was detected by error analysis.Quadratíc equation fitting of standard curve was carried out at 550 nm, which was more suitable for the detection of low-concentration xylose.[Result] This method could simultaneously detect the glucose and xylose contents in the mixed system.The recovery rates of total sugar, xylose and glucose were all between 94.00％ and 101.60％, which had relatively high accuracy.[Conclusion] This method meets the demand of saccharide determination for plant fiber hydrolysis.
关键词
总糖/葡萄糖/木糖/双波长分光光度法Key words
Total sugars/Glucose/Xylose/Dual-wavelength spectrophotometry引用本文复制引用
基金项目
江苏省高校自然科学基金(12KJD530002)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据