铜暴露对小鼠铜代谢相关酶表达及氧化应激的影响
Effects of Copper Exposure on Copper Metabolism-related Enzyme Expression and Oxidative Stress of Mice
孙倩 1马佺 2刘建军 2杨细飞3
作者信息
- 1. 深圳大学生命科学学院,广东深圳518060
- 2. 深圳市疾病预防控制中心,深圳市现代毒理学重点实验室,广东深圳518055
- 3. 深圳大学生命科学学院,广东深圳518060;深圳市疾病预防控制中心,深圳市现代毒理学重点实验室,广东深圳518055
- 折叠
摘要
[目的]研究长期低剂量铜暴露对小鼠海马组织铜含量、铜代谢相关酶及氧化应激的影响,为深入研究低剂量铜暴露的神经毒性作用提供基础数据.[方法]选择野生型小鼠,随机分为对照组和试验组对照组小鼠饮用蒸馏水,试验组在饮用水中添加0.13 mg/L氯化铜,连续饮用3个月,研究长期低剂量铜暴露对小鼠海马组织中铜含量、铜代谢相关酶表达及氧化应激的影响.[结果]与对照组相比,试验组小鼠海马中的自由铜及结合铜含量均显著升高,而铁、锌、钙离子含量在海马中均无显著差异;与对照组相比,试验组小鼠海马组织中铜蓝结合蛋白表达显著降低,蛋白硝基酪氨酸水平显著升高,8-羟基脱氧鸟嘌呤荧光表达水平显著升高.[结论]长期超低剂量铜暴露可导致铜在脑组织中聚集,但不影响铁、锌、钙和镁的含量;长期低剂量铜暴露可导致氧化应激.
Abstract
[Objective] To explore the effects of long-term low level copper exposure on copper concentration,copper metabolism-related enzyme expression and oxidative stress in mice, and to provide basic data for further exploring neurotoxicity of low level copper exposure.[Method] Widetype (WT) mice were randomly divided into the control group and test group.The mice in control group were provided with drinking water,while mice in test group were given drinking water containing 0.13 mg/L copper chloride for a period of three months.Effects of long-term copper exposure at low level on the copper concentration, oxidative stress and copper metabolism related enzymes were researched.[Result] Compared with control group ,free copper and protein binding copper were significantly increased in hippocampus of mice in test group;but the contents of iron,zinc and calcium showed no significant differences.Long-term low levels of copper exposure significantly decreased the expression level of blue copper-binding protein,but increased the levels of nitrotyrosine and 8-OHdG in hippocampus of mice.[Conclusion] Long-term copper exposure at low levels can lead to accumulation of copper but do not affect the content of iron, zinc, calcium and magnesium in the brain, and can cause oxidative stress.
关键词
铜/低剂量/海马组织/氧化应激Key words
Copper/Low level/Hippocampus/Oxidative stress引用本文复制引用
基金项目
广东省自然科学基金(2014A030313715)
深圳市科技研发基金基础研究项目(JCYJ20130329103949650)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据