药用野生稻-栽培稻渗入系后代卷叶表型的遗传分析
Phenotype Genetic Analysis of Introgression Line Progenies Rolled Leaf of O.officinais-O.sativa
柯杰 1祝贺 1覃瑞 1李刚1
作者信息
- 1. 中南民族大学南方少数民族地区生物资源保护及综合利用工程中心,湖北武汉430074
- 折叠
摘要
[目的]根据药用野生稻-栽培稻渗入系后代卷叶表型遗传分析,分析定位控制水稻叶型的相关基因.[方法]构建药用野生稻-栽培稻渗入系后代卷叶的分离群体,并对后代分离群体进行SSR多态性分析.[结果]该群体受一对不完全显性卷叶基因控制;初步判断卷叶个体表型产生的原因是野生稻基因片段的渗入所致;并将该群体的卷叶基因初步定位在第5号染色体分子标记RM305和RM173之问.[结论]得到了一个具有新来源的新基因,为进一步对新型卷叶基因的克隆和分子手段调控机理研究奠定了基础,同时为应用分子手段调控叶形来改良株型而提高水稻生产效益的设想提供材料.
Abstract
[Objective] According to the phenotype genetic analysis of introgression line progenies rolled leaf of O.officinais-O.sativa,the location of related genes controlling the leaf style of rice was studied.[Method] A segregation of O.officinais-O.sativa introgression line progenies rolled leaf was established.Moreover,SSR (Simple Sequence Repeats) polymorphic analysis was conducted to study the progenies segregation.[Result] The results showed that the group was controlled by a pair of incomplete dominance rolled leaves;the cause of rolled leaf phenotype was the infiltration of the gene segment of O.officinais;the rolled leaf genes of this group were preliminary mapped between RM305 and RM173 of molecular marker of the fifth chromosome.[Conclusion] A new gene from new source is obtained,which will lay the foundation for the further research of cloning and molecular regulation mechanism of the new rolled leaf genes and offer the possibility for increasing rice production by molecular regulation of leaf shape to improve the plant type.
关键词
渗入系/卷叶基因/基因定位/药用野生稻Key words
Introgression line/Rolled leaf gene/Gene mapping/Oryza officinalis引用本文复制引用
基金项目
国家自然科学基金(GZY15006)
中南民族大学留学基金()
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据