藏红花愈伤组织的诱导
Callus Induction of Crocus sativus L.
陈明亮 1张丽丽 1秦路平 2周志疆1
作者信息
- 1. 江苏丰收大地种业发展有限公司,江苏盐城224100
- 2. 第二军医大学,上海200433
- 折叠
摘要
[目的]建立藏红花愈伤组织诱导方法.[方法]以藏红花愈伤组织诱导率、诱导时间、外观形态作为评价参数,研究了外植体、激素种类及配比、外源添加物椰乳和活性炭浓度对藏红花愈伤组织诱导的影响.[结果]适宜的外植体是藏红花种球,适宜的激素种类及配比是MS+ KT0.5 mg,/L+ NAA 2.0 mg/L,适宜的椰乳浓度是100 mL/L,活性炭浓度是3g/L.[结论]建立的藏红花愈伤组织诱导体系稳定性好,增殖速度较快,为工厂化生产奠定基础.
Abstract
[Objective] To establish a callus induction method for Crocus sativus L.[Method] The explant ,hormone type and combination,additives (coconut milk and active carbon) concentration were optimized.The appearance ,callus induction rate and induction time were taken as the evaluation parameters.[Result] The proper explant was C.sativus seedball;proper hormone type and combination was MS + 0.5 mg/L KT +2.0 mg/L NAA.The suitable concentrations of coconut milk and active carbon in culture medium were 100 mL/L and 3 g/L,respectively.[Conclusion] This callus induction system for C.sativus has good stability and rapid multiplication rate, which lays foundation for the industrialized production.
关键词
藏红花/愈伤组织/植物激素/椰乳Key words
Crocus sativus L./Callus/Plant hormones/Coconut milk引用本文复制引用
基金项目
江苏省科学技术厅2014年度苏北科技专项资金项目(BN2014057)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据