连香树愈伤组织诱导研究
Study on Callus Induction of Cercidiphyllum japonicum Sieb.
师春娟 1王齐2
作者信息
- 1. 云南省林业科学院,云南昆明650201
- 2. 云南林业职业技术学院,云南昆明650224
- 折叠
摘要
[目的]为珍稀濒危树种连香树寻求组培快繁方法.[方法]以1/4MS、1/2MS、MS添加不同浓度的NAA、KT、GA3为培养基,以连香树当年生带芽茎段为外植体进行愈伤组织诱导.[结果]以4月份采集的带芽茎段作为外植体愈伤组织诱导效果相对较好;在1/4MS+NAA 1.0 mg/L+KT0.2 mg/L +GA3 1.00 mg/L培养基中愈伤组织诱导效果相对较好,但总体而言愈伤组织诱导率较低.[结论]连香树愈伤组织诱导具有可行性,需进一步研究.
Abstract
[Objective] The aim was to seek a way of tissue culture propagation to Cercidiphyllum japonicum.[Method] Calli were induced from young stem with a bud of C.japonicum as experimental explants, callus induction was studied on 1/4 MS,1/2 MS,MS basal medium added with different concentration hormone NAA, KT, and GA3.[Result] The effect of calli induced with explants collected in April was better than other time;and the optimum cultural medium was 1/4MS + NAA 1.0 mg/L + KT 0.2 mg/L + GA3 1.0 mg/L, as a whole, the callus induction rate was low.[Conclusion] The induction of callus is feasible and needs to be further studied.
关键词
连香树/愈伤组织/诱导Key words
Cercidiphyllum japonicum/Callus/Induction引用本文复制引用
基金项目
云南省高职高专院校提升专业服务产业能力建设项目()
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据