日照海蝉DNA条形码及其分子系统进化分析
DNA Barcoding and Molecular Evolution of Rizhao Haichan
刘丰铭 1李金泽 1梁启冰 1蔺胜娟2
作者信息
- 1. 山东省日照第一中学,山东日照276826
- 2. 山东省日照青岛路中学,山东日照276826
- 折叠
摘要
[目的]研究COⅠ基因区段作为DNA条形码在识别日照海蝉地理群体的可行性和有效性.[方法]以日照海蝉为研究对象,用引物LCO1490和HCO2198扩增线粒体COⅠ基因片段序列,产物双向测序,用Clustal W进行DNA序列比对,用MEGA 6.0软件采用邻接法(Neighbor-Joing,NJ)和最大简约法(maximum parsimony,MP)构建分子系统发育树.[结果]获得大小为658 bp的COⅠ基因,A、T、C、G的平均含量分别为30.24％、36.63％、16.41％、16.72％,其中A+T平均含量(66.87％)明显高于G+C平均含量(33.13％),NJ和MP系统发育树均表明日照海蝉属于蝉蟹总科(Hippoidea)眉足蟹科(Blepharipoda),与形态学的分属结果一致.[结论]采用COⅠ基因序列作为DNA条形码进行海蝉分类鉴定具有一定的可行性.
Abstract
[Objective] The aim was to study the feasibility and effectiveness of using COⅠ particular gene segment as the DNA barcoding in identifying Rizhao Haichan geographical group.[Method] With Rizhao Haichan as research object, the mitochondrial cytochrome C oxidase subunit Ⅰ (COⅠ) gene fragment was amplified by primers LCO1490 and HCO2198, the product was sequenced and the DNA sequence alignment was carried out by ClustalX.The phylogenetic trees were constructed using Neighbor-Joining method and Maximum Parsimony (on MEGA6.0).[Result] The COⅠ gene was found 658 bp in size, and among all the sites, the average proportion of base A, T, C and G were 30.24％, 36.63％,16.41％ and 16.72％, respectively.A + T base pair accounted for 66.87％ of the mitochondrial COⅠ gene, which was significantly higher than that of G + C base pair(33.13％).The results of NJ and MP method indicated that therelated species could gather in the same branch, and the Rizhao Haichan belongs to the family Blepharipoda in superfamily Hippoidea.[Conclusion] DNA barcoding based on mitochondrial COⅠ gene sequence is applicable in the classification and identification of Anomura.
关键词
日照海蝉/DNA条形码/COⅠ基因/系统进化Key words
Rizhao Haichan/DNA barcoding/COⅠ gene/Phylogeny引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据