铺散亚菊超临界CO2萃取挥发油成分及其抑菌活性研究
Study on the Chemical Constituents and Antibacterial Activity of the Volatile Oil from Ajania khartensis by Supercritical CO2 Fluid Extraction
达洛嘉 1马家麟 1白雪 1高世宝 1林鹏程1
作者信息
- 1. 青海民族大学药学院,青海省青藏高原植物化学重点实验室,青海省药物分析学重点实验室,青海西宁810007
- 折叠
摘要
[目的]研究藏药铺散亚菊挥发油的主要成分及其抑菌活性.[方法]采用超临界CO2法萃取铺散亚菊挥发油成分,采用气相色谱质谱联用(CC-MS)结合相对保留指数进行组分分析鉴定,并通过滤纸片扩散法筛选铺散亚菊挥发油对常见13种致病菌的抑菌活性.[结果]从铺散亚菊超临界CO2萃取物中共分离出127个可识别峰,含量高于0.02％的化学成分为46种,占总萃取物的67.30％.铺散亚菊挥发油对乙型副伤寒沙门菌、产气肠杆菌等11种供试菌表现出抑菌活性.[结论] GC-MS结合保留指数对铺散亚菊超临界CO2萃取挥发油进行定性分析,使定性结果更为可靠.该研究结果可为铺散亚菊挥发油的抗菌活性研究提供试验依据.
Abstract
[Objective] To research the chemical components of volatile oil from Ajania khartensis,and to investigate their antimicrobial activities.[Method] The volatile oil from Ajania khartensis was obtained by supercritical CO2 fluid extraction,then combined GC-MS analysis with retention index to appraise the chemical components,and screened antimicrobial activities of the volatile to thirteen common bacterial pathogens by filter paper diffusion method.[Result] 127 identifiable peaks were isolated from the volatile oil,among them,46 components' relative content were higher than 0.02％ which accounted for 67.30％ of the total extract.The volatile oil from Ajania khartensis showed some antimicrobial activities against Salmonella paratyphy B and Enterobacter aerogenes.[Conclusion] It ensures the qualitative results by using GC-MS and retention index to analyze the chemical components of volatile oil from Ajania khartensis.The filter paper diffusion method provides a reliable preparatory screening result for antimicrobial activities of volatile oil from Ajania khartensis.
关键词
铺散亚菊/超临界CO2萃取/GC-MS/抑菌活性Key words
Ajania khartensis/Supercritical CO2 fluid extraction/GC-MS/Antibacterial activity引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
万方数据