摘要
[目的]了解我国地方鸡种禽白血病的自然感染状态,为今后该病的净化提供依据.[方法]采用ELISA法和病毒分离方法,对国内10个地方鸡种开展了ALV-AB和ALV-J主要外源性禽白血病流行病学调查,并进一步对其中4个品系的F2代鸡群进行ALV跟踪监测,同时对饲养过程中使用的弱毒活疫苗也进行了ALV抽样检测.[结果]ALV-P27抗原阳性率为0～62.1％,ALV-AB抗体阳性率为0 ～25.0％,ALV-J抗体阳性率为0～ 59.0％,病毒分离阳性率为0～22.0％.4个品系的F2代鸡群1日龄胎粪ALV-P27抗原阳性率为6.0％～67.0％,6周龄病毒分离检测阳性率为2.0％～34.3％;国内有1个厂家2个种类2个批次的疫苗分离有ALV.[结论]我国地方鸡种普遍存在ALV感染,不同地方鸡种间差异较大,可能与不同地方鸡种对ALV的遗传抗性有关;某些品系种群的F2代雏鸡ALV阳性率甚至略有提高,可能与疫苗接种污染有关.
Abstract
[Objective] To find the nature infection status of avian leukemia in some indigenous chicken.[Method] By using the method of ELISA method and virus isolation method,we investigated the ALV-AB and ALV-J avian leukemia of ten indigenous chicken.ALV monitoring was carried out by the second generation of chickens in four lines.ALV sampling inspection was adopted for the modified-live vaccine in raising process.[Result] The ALV-P27 antigen positive rate was 0-62.1％ ; ALV-AB antibody positive rate was 0-25.0％ ; ALV-J antibody positive rate was 0-59.0％ ; positive rate of virus separation was 0-22.0％.ALV-P27 antigen positive rate from the offsprings of four lines was 6.0％ -67.0％.Positive rate of virus isolation test was 2.0％-34.3％ at the age of six-week old.Two kinds of vaccines from two batches produced by a manufacturer were polluted by ALV.[Conclusion] Most indigenous chicken are infected by ALV.There are great differences among the different breeds of indigenous chicken,which might be related to the ALV genetic resistance of different indigenous chicken.ALV positive rate of F2 chicks have enhanced slightly in some populations,which might be related to the vaccination pollution.
基金项目
公益性行业(农业)科研专项(201203055)
江苏省农业三新工程项目(SXGC[2014] 288)
江苏省自然科学基金(20151317)
扬州市科技计划项目(YZ2014144)
国家科技期刊平台
NSTL
维普
万方数据