甘草黄酮对UVB损伤HDF细胞保护作用研究
Effects of Licoflavone on HDF Cytoprotection after UVB Injury
付维维 1刘娟 2王业秋 3李艳微 3徐艳明 3张宁 3李建民3
作者信息
- 1. 佳木斯大学,黑龙江佳木斯154007;黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007
- 2. 佳木斯大学,黑龙江佳木斯154007
- 3. 黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007
- 折叠
摘要
[目的]研究甘草黄酮对UVB照射后产生光老化现象的HDF细胞保护作用.[方法]采用30 mJ/cm2的UVB照射HDF细胞建立损伤模型;加入不同浓度的甘草黄酮,24h后MTT法检测细胞活性;用试剂盒法测定细胞上清液中谷胱甘肽过氧化物酶(GSH-Px)、起氧化物歧化酶(SOD)活性、丙二醛(MDA)含量.[结果]甘草黄酮浓度为10-9、10-10、10-11 mol/L时,能使受UVB照射损伤的HDF细胞活性增强;甘草黄酮浓度为10-9、10-10 mol/L时,GSH-Px、SOD活性升高,MDA含量降低;甘草黄酮浓度为10-11 mol/L时,SOD活性升高、MDA含量降低.[结论]甘草黄酮能减轻UVB对HDF细胞的损伤,其机制可能与抑制氧化损伤、提高SOD活性有关.
Abstract
[Objective] To research the effects of licoflavone on HDF cytoprotection after UVB injury.[Method] HDF was irradiated under 30 mJ/cm2 UVB to establish injury mode.After adding different concentrations of licoflavone,cell viability was detected by MTT method after 24 h.Contents of GSH-Px,SOD and MDA in cell supernatant were detected by kit method.[Result] When licoflavone concentration was 10-9,10-10 and 10-11 mol/L,HDF activities enhanced after UVB injury.When licoflavone content was 10-9 and 10-10 mol/L,contents of GSH-Px,SOD and MDA reduced.When licoflavone content was 10-11 mol/L,SOD activity enhanced and MDA content reduced.[Conclusion] Licoflavone reduces the HDF injury induced by UVB.Its mechanism might be related to the restriction of oxidative damage and the enhancement of SOD.
关键词
甘草黄酮/光老化/UVB/HDF细胞/保护作用Key words
Licoflavone/Photoaging/UVB/HDF cell/Protection effects引用本文复制引用
基金项目
黑龙江省应用技术研究与开发计划项目(PC13S15)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据